Mo has a Go

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GMO
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Mo has a Go

Post by GMO »

So after growing Lions mane along with @Nanook Ive take the leap to go from spore syringe

Imma be doing both BRF (Brown Rice Flour) and Uncle Ben's Tek

Kit

ImageImageImageImageImage

SAB (still air box)
3x jam jar with
2:1 vermiculite:BRF
2x Uncle ben's wholegrain pouches
Gloves
Disinfectant wipes/spray
70% Iso wipes/spray
Microporous tape
Blow torch (for sterilizing syringe)
Spores of your choice

So I began by prepping everything, gently broke up the rice pouch and force the rice down to the bottom

Image

Hammered 4 holes at the compass points into the jar lids prior to filling and sterilizing. I then cut pieces of tape to cover the intended injection holes and put them next to each hole in the lid and on at the bottom and one near the top of the Uncle Ben's pouches

Using the torch to heat the needle tip red hot for 10 seconds prior to initial inoculation as well as between inoculation and then once more before returning the safety cap should hopefully keep me nice and sterile. 1/4th 1ml per hole in the jar lids = 1ml per jar. I immediately cover the holes with the tape and after all 4 are done i reapply the foil used during sterilizing

With the pouch; I injected 1ml through the coloured front of the packaging near the base on the clear section at the bottom (being very careful not to pierce the bottom clear section. again cover the hole immediately. i think move to the top corner and make a surface peircing by pinching the front surface to raise it allowing me to push the needle tip in and out the same side. This is not the air exchange and once again is immediately covered the tape

finally i just date everything and put it somewhere i can forget it for 10 days or so

Image

I have shortened the first few steps a bit. the jars were sterilized twice in a 15psi pressure cooker for 80 mins per cooking and everything was thoroughly cleaned with the disinfectant followed by the alcohol to help keep everything super clean (thats hand, gloves, pouches, syringe, torch, EVERYTHING clean and feel free to clean it again to be sure!)

Image

Wish me luck and any advice happily received
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Re: Mo has a Go

Post by Marcus »

Hi MO.

Don;t cover the holes with tape or foil. You should have a top centimetre of dry vermiculite in those jars that act as a breathable filter.

You don;t say if you added water to the brf cakes and at what ratio?

They only need once in the PC. You can store them till needed then, foil on.

:oik!:

Good luck. :drama:
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Re: Mo has a Go

Post by GMO »

Marcus wrote:
Mon Nov 09, 2020 12:32 pm
Hi MO.

Don;t cover the holes with tape or foil. You should have a top centimetre of dry vermiculite in those jars that act as a breathable filter.

You don;t say if you added water to the brf cakes and at what ratio?

They only need once in the PC. You can store them till needed then, foil on.

:oik!:

Good luck. :drama:
I do have the vermi top on the cake. just talking to Nom at the time of doing it, tape and foil was what he also did. He also cooks 3 times, i went for twice cos i was gonna do them, then realized i didnt have alcohol wipes so cooked them again so they would be fresh ready for when i was inoculating :idn: I just joined all the advise i had been given and was reading

The water ratio i went about the other way, i added 2 table spoons of water and stirred and repeated until everything was evenly moist to the point if squeezed barely a drip would come out :oik!:
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Re: Mo has a Go

Post by Dandy Dan »

I only cooked 3 times because I was super paranoid about contamination so i went OTT with it, Marcus is correct once is enough and the dry vermiculite is your barrier against contamination after innoculation. It's been a long time since I've done this though
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Re: Mo has a Go

Post by Nanook »

I thought uncle bens was banned now :o
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Re: Mo has a Go

Post by GMO »

why would uncle bens be banned?

after the above convo about covering the holes in the lid; i removed the tape from 2 and replaced the tinfoil on one. so we have 1x uncovered 1x just tin foil and 1x tin foil and tape :oik!:

report back in a fortnight or so :bye:
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Re: Mo has a Go

Post by Nanook »

Apparently its racist.... tbh I just saw a black man on a bag of rice, never even considered it that way.
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Re: Mo has a Go

Post by GMO »

Day 4 in da big mushroom house (said in a northern accent)

Uncle ben has been a very naughty boy

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And the triplets arent far behind

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Re: Mo has a Go

Post by Josh »

Nanook wrote:Apparently its racist.... tbh I just saw a black man on a bag of rice, never even considered it that way.
It's only racist to white male-feminists sry to jack your thread gmo, just find the cancel culture thing hilarious

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Re: Mo has a Go

Post by Nanook »

Lol josh. It reminds me of southpark flag and the kids just saw a bloke hanging. .... the worlds gone mad!
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Re: Mo has a Go

Post by GMO »

Few days later

ImageImage
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Re: Mo has a Go

Post by Nanook »

wow! looking great gmo :)
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Re: Mo has a Go

Post by Josh »

How long is it supposed to take gmo?

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Re: Mo has a Go

Post by GMO »

Josh wrote:
Sun Nov 15, 2020 10:03 pm
How long is it supposed to take gmo?

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Good question :idn:

a quick google turns up; 3-4 weeks to colonize and about a week to fruit (plus a week or so for issues) - So apparently roughly 6 weeks :idn:
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Re: Mo has a Go

Post by GMO »

Josh wrote:
Sun Nov 15, 2020 10:03 pm
How long is it supposed to take gmo?

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Also
Uncle bens Tek
Break up the mycelium chunks to spread mycelium covered grain evenly throughout the bag and return the bags to the dark spawning area. Once the bag is fully colonized (12-30 days) you may either place into fruiting condition or prepare your spawn for a bulk grow
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Re: Mo has a Go

Post by GMO »

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Sexy sexy
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Re: Mo has a Go

Post by DIY.Rik »

That's pretty insane to look at to be fair. Nice one @GMO
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Re: Mo has a Go

Post by GMO »

some random pics

ImageImageImage

:rock: :rock:


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:dance:
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Re: Mo has a Go

Post by camkush »

You are my favorite scientist 🤙🏻😎
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Re: Mo has a Go

Post by GMO »

Image

hopefully fully colonized tomorrow, then 7 days of consolidation
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